19/10/2026
EL ALMACENAMIENTO DE SEMEN CONGELADO
Articulo publicado en Journal of Equine Veterinary Science 145:105332. https://doi.org/10.1016/j.jevs.2024.105332)
Conclusiones del estudio
•La calidad es***mática convencional no se ve afectada de manera significativa por el almacenamiento en nitrógeno líquido durante un período de hasta 26 años.
•La abundancia de ARN mensajero asociado con la fertilidad disminuye con el aumento del tiempo de almacenamiento en determinados genes candidatos.
•Los principales cambios en la calidad es***mática se producen durante el proceso de criopreservación, y no durante el almacenamiento posterior.
•Se necesitan muestras de mayor tamaño para confirmar los hallazgos relacionados con el ARN mensajero.
💥𝐈𝐒𝐒𝐑 𝐒𝐧𝐢𝐩𝐩𝐞𝐭!🐴 𝐋𝐨𝐧𝐠-𝐓𝐞𝐫𝐦 𝐋𝐢𝐪𝐮𝐢𝐝 𝐍𝐢𝐭𝐫𝐨𝐠𝐞𝐧 𝐒𝐭𝐨𝐫𝐚𝐠𝐞 𝐄𝐟𝐟𝐞𝐜𝐭𝐬 𝐨𝐧 𝐅𝐫𝐨𝐳𝐞𝐧 𝐒𝐭𝐚𝐥𝐥𝐢𝐨𝐧 𝐒𝐞𝐦𝐞𝐧 𝐐𝐮𝐚𝐥𝐢𝐭𝐲 𝐚𝐧𝐝 𝐌𝐞𝐬𝐬𝐞𝐧𝐠𝐞𝐫 𝐑𝐍𝐀 𝐀𝐛𝐮𝐧𝐝𝐚𝐧𝐜𝐞
Cryopreserved semen stored in liquid nitrogen is widely assumed to maintain fertility indefinitely, yet emerging reports suggest possible long-term quality decline. This study examined whether extended storage duration affects conventional s***m quality parameters and fertility-associated messenger RNA abundance in commercially cryopreserved stallion semen.
Study Design:
• 18 stallions (Warmblood, Thoroughbred, Lipizzaner); semen cryopreserved between 1998 and 2024; storage duration 0–26 years
• Post-thaw assessment: concentration, motility, membrane integrity, mitochondrial membrane potential, and chromatin condensation
• Messenger RNA extracted from 12 semen batches; abundance of 10 fertility-associated genes measured by quantitative reverse transcription polymerase chain reaction
• Spearman correlation analysis performed between all endpoints and storage duration
Key Results:
• No significant correlations between storage duration and any conventional s***m quality parameter
• No significant correlations between storage duration and messenger RNA quality metrics
• Four genes showed significant negative correlations with storage duration:
•• Protamine 1 (r=–0.338)
•• Protamine 3 (r=–0.373)
•• Peroxiredoxin 6 (r=–0.336)
•• Cysteine-rich secretory protein 3 (r=–0.507)
Conclusions:
• Conventional s***m quality is not meaningfully affected by liquid nitrogen storage for up to 26 years
• Fertility-associated messenger RNA abundance does decline with extended storage duration for select candidate genes
• Major s***m quality changes occur at cryopreservation processing, not during subsequent storage
• Larger sample sizes are needed to confirm messenger RNA findings
(Vigolo V, Ertl R, Aurich C. 2025. Long-term storage does not result in considerable changes of frozen stallion semen characteristics but affects semen mRNA abundance. Journal of Equine Veterinary Science 145:105332. https://doi.org/10.1016/j.jevs.2024.105332)