Dr. Abhinaba Ghosh

Dr. Abhinaba Ghosh MBBS, MSc, PhD (distinction)
Physician & Scientist Immunoblot: Western Blot technique (brief experience). Different antigen-antibody reactions.

Google Scholar Profile:

https://scholar.google.ca/citations?hl=en&user=edE1D0YAAAAJ


Research Highlights and Media Attention:

 Ottawa invests $3.7M in research projects at MUN | SaltWire

https://www.saltwire.com/atlantic-canada/business/local-business/ottawa-invests-37m-in-research-projects-at-mun-291647/

 March 2019 Gazette - Memorial University of Newfoundland

https://gazette.mun.ca/rese

arch/energize-and-enhance/attachment/researchfunding_20190312_099/

 Critical role (mun.ca)

https://gazette.mun.ca/research/critical-role-2/

 Interview November 2021, Canada

https://www.grenfell.mun.ca/academics-and-research/Pages/Aging-Research-Centre/Funding-Opportunities/Abhinaba-Ghosh.aspx



Research Skills:

Slice electrophysiology

Whole cell patch clamp recordings measuring intrinsic and synaptic properties
Whole cell calcium current recording
Brief experience in dendritic calcium imaging in dendrites in piriform cortex
Data analysis using Clampfit. Loose patch extra cellular recording from whole-mounted retina/ retinal slices

Behavioral experiments

Experienced in animal handling (rat, mouse, guinea pig, frog)
In vivo optogenetic and chemogenetic modulation of behavioral tests
Olfactory preference learning: Y-maze, olfactometer, food retrieval paradigm Aversive olfactory learning: odor-shock conditioning
Neonatal preference learning by odor-stroke model Elevated plus maze
Visual cliff test

Molecular Biology

Immunohistochemistry: fluorescence immunostaining, double Immunostaining. Microscopy/imaging

Wide field optical mapping: In vivo cortical imaging of calcium and norepinephrine signal. Light microscopy, fluorescence microscopy, LASER scanning confocal microscopy; analysis in ImageJ. Surgical Procedures

Stereotaxic brain surgery: infusion, cannula implantation in adult and neonatal rodents Cortical window placement surgery for wide field optical mapping
Transcardiac perfusion and brain dissection Eyeball and acute retina extraction under microscope
Fixed brain tissue slicing in cryotome and vibrotome


Programing experience

MATLAB (wrote new codes and customized existing codes for imaging data analysis)


Public Health / Disease Informatics

Co-developed assessment tool for stroke care services in India, contributed in improving data input system for hospital based stroke registry, beta-tested software system liaising with IT team, visited hospitals auditing death certification system and corresponding data acquisition. Present and past professional memberships

 Canadian Association of Neuroscience (CAN)
 Society for Neuroscience (SFN)
 Aging Research Center-Newfoundland and Labrador (ARC-NL)
 Life member, Indian Medical Association


Administrative Service

 Coordinator of electrophysiology journal club; Memorial University of Newfoundland (December 2018- December 2019)
 Reviewer for Springer-Nature group’s journal Scientific Reports 2021
 Reviewer for Elsevier group’s journal Neuropharmacology 2022

Address

Kolkata

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