19/01/2025
๐พ๐ผ๐๐ ๐๐๐จ๐ฉ๐
โช๏ธIt is used for the identification of ๐๐ฉ๐ง๐๐ฅ๐ฉ๐ค๐๐ค๐๐๐ช๐จ ๐๐๐๐ก๐๐๐ฉ๐๐๐.
โข It is a Group ๐ ๐๐๐ญ๐-๐ก๐๐ฆ๐จ๐ฅ๐ฒ๐ญ๐ข๐ streptococci.
โข It is the only beta-hemolytic Streptococcus which secrete a protein called ๐๐๐๐ ๐๐๐๐ญ๐จ๐ซ ๐จ๐ซ "๐ฉ๐ซ๐จ๐ญ๐๐ข๐ง ๐".
โข It is one of the causative agents of ๐ฆ๐๐ฌ๐ญ๐ข๐ญ๐ข๐ฌ ๐ข๐ง ๐๐จ๐ฐ๐ฌ.
โช๏ธ ๐พ๐ผ๐๐ is an acronym for the authors of this test (๐๐ก๐ซ๐ข๐ฌ๐ญ๐ข๐, ๐๐ญ๐ค๐ข๐ง๐ฌ๐จ๐ง, ๐๐ฎ๐ง๐๐ก, ๐๐ง๐ ๐๐๐ญ๐๐ซ๐ฌ๐จ๐ง) which was identified in 1944.
๐ฃ๐ฟ๐ถ๐ป๐ฐ๐ถ๐ฝ๐น๐ฒ๐
โข Streptococcus agalactiae produces a diffusible, thermostable, extracellular protein known as CAMP factor.
โช๏ธCAMP factor acts synergistically with the beta lysin produced by Staphylococcus aureus to produce a zone of enhanced lysis of sheep or bovine erythrocytes.
โข The group B streptococci are streaked ๐ฉ๐๐ซ๐ฉ๐๐ง๐๐ข๐๐ฎ๐ฅ๐๐ซ ๐ญ๐จ ๐ ๐ฌ๐ญ๐ซ๐๐๐ค ๐จ๐ ๐. ๐๐ฎ๐ซ๐๐ฎ๐ฌ on sheep blood agar.
๐ฅ๐ฒ๐๐๐น๐๐๐
โข A ๐ฉ๐จ๐ฌ๐ข๐ญ๐ข๐ฏ๐ ๐ซ๐๐๐๐ญ๐ข๐จ๐ง appears as an ๐๐ง๐ก๐๐ง๐๐๐ ๐ก๐๐ฆ๐จ๐ฅ๐ฒ๐ฌ๐ข๐ฌ is indicated by an arrow head-shaped zone of beta-hemolysis at the junction of the two organisms.
โข Negative: No ๐๐ง๐ก๐๐ง๐๐๐ฆ๐๐ง๐ญ ๐จ๐ ๐ก๐๐ฆ๐จ๐ฅ๐ฒ๐ฌ๐ข๐ฌ.
๐๐จ๐๐จ ๐ค๐ ๐พ๐ผ๐๐ ๐ฉ๐๐จ๐ฉ๐
โช๏ธ It can be used to determine the ability of an organism to produce the CAMP factor.
โช๏ธ It is used to differentiate Streptococcus agalactiae from other species of beta-hemolytic Streptococcus.
โช๏ธIt is used to identify Listeria monocytogenes which also produces a positive CAMP reaction.