Equine-Reproduction.com LLC

Equine-Reproduction.com LLC An Equine Reproduction Management & Educational Service providing information and services worldwide! CE accredited courses for veterinarians and vet. techs.

Equine-Reproduction.com is dedicated to providing both basic and advanced information to horse breeders world-wide. The site is used as a resource by many Universities in their equine reproduction programs, and all information offered on the site is free with no restrictions for viewing. Equine reproduction short courses, semen freezing and stallion/mare management services are also offered throug

h the site in many different locations throughout the USA. are available, as well as courses suitable for the general public.

We regularly update articles on the website to ensure current research and trends are represented. Our "oxytocin protoco...
08/31/2026

We regularly update articles on the website to ensure current research and trends are represented. Our "oxytocin protocol" article dates back over 25 years to close to the original creation date of the website and has assisted many breeders to get their mares pregnant. It's undergone several updates in that time, and today we added another! There are no changes to the protocol itself, but we have updated research references to include newer peer-reviewed paper citations, as well as discussing some alternative drugs and factors related to DUC/PBIE - take a look!!

https://Equine-Reproduction.com/oxytocin

๐’๐ž๐ฆ๐ž๐ง ๐€๐ฌ๐ฌ๐ž๐ฌ๐ฌ๐ฆ๐ž๐ง๐ญ ๐š๐ฌ ๐š ๐…๐ž๐ซ๐ญ๐ข๐ฅ๐ข๐ญ๐ฒ ๐๐ซ๐ž๐๐ข๐œ๐ญ๐จ๐ซ ๐ข๐ง ๐’๐ญ๐š๐ฅ๐ฅ๐ข๐จ๐ง๐ฌIn equine reproduction we routinely use s***m motility scores - to...
08/31/2026

๐’๐ž๐ฆ๐ž๐ง ๐€๐ฌ๐ฌ๐ž๐ฌ๐ฌ๐ฆ๐ž๐ง๐ญ ๐š๐ฌ ๐š ๐…๐ž๐ซ๐ญ๐ข๐ฅ๐ข๐ญ๐ฒ ๐๐ซ๐ž๐๐ข๐œ๐ญ๐จ๐ซ ๐ข๐ง ๐’๐ญ๐š๐ฅ๐ฅ๐ข๐จ๐ง๐ฌ

In equine reproduction we routinely use s***m motility scores - total and progressive - as markers for "insemination quality" even though it is well-documented that motility is not necessarily correlated to fertility (i.e. one can have good motility and poor or no fertility). While clearly motility is of some importance (zero motility is rarely a good starting point!) alternative evaluation methods pointing towards fertility chances such as flow-cytometry are often expensive and impractical, particularly for the low-budget or in-the-field operator. This week's "Snippet" from the 8th International Symposium on Stallion Reproduction presents Amorim et al's review of a cheap and effective alternative in morphological evaluation:

https://equine-reproduction.com/articles/issr-8/semen-assessment-as-a-fertility-predictor

We share the summary on our website, courtesy of and with the permission of the International Society for Equine Reproduction.

(Amorim GBAG, Oliveira OM, Papa FO, Dell'Aqua Jr JA, Alvarenga MA, Freitas-Dell'Aqua CP. 2025. Can a simple and cheap semen assessment help with fertility? JEVS 145:105262 - Abstract available on-line at https://doi.org/10.1016/j.jevs.2024.105262)

๐๐ซ๐จ๐ฌ๐ญ๐š๐ง๐จ๐ข๐๐ฌ ๐š๐ฌ ๐Œ๐š๐ซ๐ค๐ž๐ซ๐ฌ ๐จ๐Ÿ ๐Ž๐ฑ๐ข๐๐š๐ญ๐ข๐ฏ๐ž ๐’๐ญ๐ซ๐ž๐ฌ๐ฌ ๐ข๐ง ๐’๐ญ๐š๐ฅ๐ฅ๐ข๐จ๐ง ๐’๐ž๐ฆ๐ž๐งStandard pre-processing semen assessment does not reliably p...
08/15/2026

๐๐ซ๐จ๐ฌ๐ญ๐š๐ง๐จ๐ข๐๐ฌ ๐š๐ฌ ๐Œ๐š๐ซ๐ค๐ž๐ซ๐ฌ ๐จ๐Ÿ ๐Ž๐ฑ๐ข๐๐š๐ญ๐ข๐ฏ๐ž ๐’๐ญ๐ซ๐ž๐ฌ๐ฌ ๐ข๐ง ๐’๐ญ๐š๐ฅ๐ฅ๐ข๐จ๐ง ๐’๐ž๐ฆ๐ž๐ง

Standard pre-processing semen assessment does not reliably predict quality after chilled storage. Oxidative stress in stallion semen during cooling damages s***m membranes via lipid peroxidation, reducing fertilizing potential. This study investigated whether seminal plasma prostanoids โ€” established lipid peroxidation biomarkers โ€” could predict chilled semen quality in stallions.

We share the summary on our website, courtesy of and with the permission of the International Society for Equine Reproduction.

(Gallacher K, Noschka E, Mihm Carmichael M, Rozek T, Galano JM, Durand T, Oger C, Barnes SA. 2025. Prostanoids as markers of oxidative stress in stallion semen. Journal of Equine Veterinary Science 145:105287 - Abstract available on-line at https://doi.org/10.1016/j.jevs.2024.105287)

๐“๐ž๐ฌ๐ญ๐ข๐œ๐ฎ๐ฅ๐š๐ซ ๐š๐ง๐ ๐„๐ฉ๐ข๐๐ข๐๐ฒ๐ฆ๐š๐ฅ ๐†๐ž๐ง๐ž ๐„๐ฑ๐ฉ๐ซ๐ž๐ฌ๐ฌ๐ข๐จ๐ง ๐‚๐ก๐š๐ง๐ ๐ž๐ฌ ๐ข๐ง ๐‘๐ž๐ฌ๐ฉ๐จ๐ง๐ฌ๐ž ๐ญ๐จ ๐’๐œ๐ซ๐จ๐ญ๐š๐ฅ ๐‡๐ž๐š๐ญ๐ข๐ง๐  ๐ข๐ง ๐’๐ญ๐š๐ฅ๐ฅ๐ข๐จ๐ง๐ฌHeat stress is a well-recog...
08/08/2026

๐“๐ž๐ฌ๐ญ๐ข๐œ๐ฎ๐ฅ๐š๐ซ ๐š๐ง๐ ๐„๐ฉ๐ข๐๐ข๐๐ฒ๐ฆ๐š๐ฅ ๐†๐ž๐ง๐ž ๐„๐ฑ๐ฉ๐ซ๐ž๐ฌ๐ฌ๐ข๐จ๐ง ๐‚๐ก๐š๐ง๐ ๐ž๐ฌ ๐ข๐ง ๐‘๐ž๐ฌ๐ฉ๐จ๐ง๐ฌ๐ž ๐ญ๐จ ๐’๐œ๐ซ๐จ๐ญ๐š๐ฅ ๐‡๐ž๐š๐ญ๐ข๐ง๐  ๐ข๐ง ๐’๐ญ๐š๐ฅ๐ฅ๐ข๐จ๐ง๐ฌ

Heat stress is a well-recognized cause of testicular and epididymal dysfunction, yet the underlying molecular events driving these changes remain poorly understood. This study used transcriptomic analysis to characterize early gene expression responses in testicular and epididymal tissues following acute scrotal insulation in stallions.

This week's "Snippet" from the 8th International Symposium on Stallion Reproduction reviews the actual changes that occur at a cellular level to cause those heat-related s***m issues.

https://equine-reproduction.com/articles/issr-8/changes-in-response-to-scrotal-heating

We share the summary on our website, courtesy of and with the permission of the International Society for Equine Reproduction.

(Ferrer M, Barletta M, Williams J, Moran K, Bilbao G, Bartolome J. 2025. Changes in equine testicular and epididymal homeostasis in response to scrotal heating. JEVS 145:105284 - Abstract available on-line at https://doi.org/10.1016/j.jevs.2024.105284)

๐ˆ๐ง๐œ๐ซ๐ž๐š๐ฌ๐ข๐ง๐  ๐’๐ฉ๐ž๐ซ๐ฆ ๐Œ๐ž๐ฆ๐›๐ซ๐š๐ง๐ž ๐‚๐ก๐จ๐ฅ๐ž๐ฌ๐ญ๐ž๐ซ๐จ๐ฅ ๐ˆ๐ฆ๐ฉ๐ซ๐จ๐ฏ๐ž๐ฌ ๐‚๐จ๐ฅ๐ ๐’๐ก๐จ๐œ๐ค ๐‘๐ž๐ฌ๐ข๐ฌ๐ญ๐š๐ง๐œ๐ž ๐š๐ง๐ ๐‚๐ซ๐ฒ๐จ๐ฌ๐ฎ๐ซ๐ฏ๐ข๐ฏ๐š๐ฅ ๐ข๐ง ๐’๐ญ๐š๐ฅ๐ฅ๐ข๐จ๐ง๐ฌIt is well known that ...
08/05/2026

๐ˆ๐ง๐œ๐ซ๐ž๐š๐ฌ๐ข๐ง๐  ๐’๐ฉ๐ž๐ซ๐ฆ ๐Œ๐ž๐ฆ๐›๐ซ๐š๐ง๐ž ๐‚๐ก๐จ๐ฅ๐ž๐ฌ๐ญ๐ž๐ซ๐จ๐ฅ ๐ˆ๐ฆ๐ฉ๐ซ๐จ๐ฏ๐ž๐ฌ ๐‚๐จ๐ฅ๐ ๐’๐ก๐จ๐œ๐ค ๐‘๐ž๐ฌ๐ข๐ฌ๐ญ๐š๐ง๐œ๐ž ๐š๐ง๐ ๐‚๐ซ๐ฒ๐จ๐ฌ๐ฎ๐ซ๐ฏ๐ข๐ฏ๐š๐ฅ ๐ข๐ง ๐’๐ญ๐š๐ฅ๐ฅ๐ข๐จ๐ง๐ฌ

It is well known that stallion s***m are temperature-sensitive. This is manifested in a variety of ways connected to both cooling and freezing of semen, typically with a negative outcome.

This week's "Snippet" from the 8th International Symposium on Stallion Reproduction reviews the benefits of Cholesterol to protect stallion s***m from both cold shock and cryopreservation damage.

https://equine-reproduction.com/articles/issr-8/cholesterol-improves-cold-shock-resistance

We share the summary on our website, courtesy of and with the permission of the International Society for Equine Reproduction.

(Cittone M, Razquin P, Graham J. 2025. Increasing stallion s***m membrane cholesterol improves cold shock resistance and cryosurvival of stallion s***m. JEVS 145:105276 - Abstract available on-line at https://www.sciencedirect.com/science/article/abs/pii/S073708062400282X)

๐“๐ก๐ž ๐„๐Ÿ๐Ÿ๐ž๐œ๐ญ๐ฌ ๐จ๐Ÿ ๐ƒ๐ข๐Ÿ๐Ÿ๐ž๐ซ๐ž๐ง๐ญ ๐๐ซ๐ž-๐…๐ซ๐ž๐ž๐ณ๐ž ๐‚๐ž๐ง๐ญ๐ซ๐ข๐Ÿ๐ฎ๐ ๐š๐ญ๐ข๐จ๐ง ๐„๐ฑ๐ญ๐ž๐ง๐๐ž๐ซ ๐ˆ๐ง๐ ๐ซ๐ž๐๐ข๐ž๐ง๐ญ๐ฌ ๐จ๐ง ๐…๐ข๐ง๐š๐ฅ ๐Ž๐ฎ๐ญ๐œ๐จ๐ฆ๐žTypically, preparation of semen ...
07/25/2026

๐“๐ก๐ž ๐„๐Ÿ๐Ÿ๐ž๐œ๐ญ๐ฌ ๐จ๐Ÿ ๐ƒ๐ข๐Ÿ๐Ÿ๐ž๐ซ๐ž๐ง๐ญ ๐๐ซ๐ž-๐…๐ซ๐ž๐ž๐ณ๐ž ๐‚๐ž๐ง๐ญ๐ซ๐ข๐Ÿ๐ฎ๐ ๐š๐ญ๐ข๐จ๐ง ๐„๐ฑ๐ญ๐ž๐ง๐๐ž๐ซ ๐ˆ๐ง๐ ๐ซ๐ž๐๐ข๐ž๐ง๐ญ๐ฌ ๐จ๐ง ๐…๐ข๐ง๐š๐ฅ ๐Ž๐ฎ๐ญ๐œ๐จ๐ฆ๐ž

Typically, preparation of semen for freezing requires that it be centrifuged to concentrate the s***m and reduce the overall volume, allowing addition of freezing extender. Although the centrifugation extender is mostly removed prior to that addition, the s***m are exposed to the centrifugation extender for a varying amount of time depending upon the protocol being used.

This week's "Snippet" from the 8th International Symposium on Stallion Reproduction reviews the effects of different extender ingredients on the freezing outcomes.

https://equine-reproduction.com/articles/issr-8/pre-freeze-semen-centrifugation-extenders

We share the summary on our website, courtesy of and with the permission of the International Society for Equine Reproduction.

(de Zutter BM, Freitas-Dellโ€™Aqua CP, Dellโ€™Aqua Jr JA, Trinque CM, Frasson MS, Canuto LEF, Troncarelli T, Monteiro GA, Alvarenga MA, Papa FO. 2025. The role of different extenders in pre-centrifugation for optimizing equine semen cryopreservation. JEVS 145:105339 - Abstract available on-line at https://www.sciencedirect.com/science/article/abs/pii/S0737080624003459)

๐’๐ก๐ข๐ฉ๐ฉ๐ข๐ง๐  ๐’๐ž๐ฆ๐ž๐ง ๐Ÿ๐จ๐ซ ๐…๐ซ๐ž๐ž๐ณ๐ข๐ง๐  ๐„๐ฅ๐ฌ๐ž๐ฐ๐ก๐ž๐ซ๐ž - ๐€๐ซ๐ž ๐€๐ฅ๐ฅ ๐„๐ฑ๐ญ๐ž๐ง๐๐ž๐ซ๐ฌ ๐‚๐ซ๐ž๐š๐ญ๐ž๐ ๐„๐ช๐ฎ๐š๐ฅ๐ฅ๐ฒ?It is possible to have a stallion collected an...
07/20/2026

๐’๐ก๐ข๐ฉ๐ฉ๐ข๐ง๐  ๐’๐ž๐ฆ๐ž๐ง ๐Ÿ๐จ๐ซ ๐…๐ซ๐ž๐ž๐ณ๐ข๐ง๐  ๐„๐ฅ๐ฌ๐ž๐ฐ๐ก๐ž๐ซ๐ž - ๐€๐ซ๐ž ๐€๐ฅ๐ฅ ๐„๐ฑ๐ญ๐ž๐ง๐๐ž๐ซ๐ฌ ๐‚๐ซ๐ž๐š๐ญ๐ž๐ ๐„๐ช๐ฎ๐š๐ฅ๐ฅ๐ฒ?

It is possible to have a stallion collected and the semen shipped cooled to a different location for freezing elsewhere. It has previously been documented that "not all semen extenders work for all stallions" and pre-breeding-season evaluations in different extenders is an important part of setting up a responsible cooled semen shipping program. So does that also apply to semen which is to be subsequently frozen?

This week's "Snippet" from the 8th International Symposium on Stallion Reproduction reviews the effects of four different Botupharma extenders on the freezing outcomes.

https://equine-reproduction.com/articles/issr-8/effect-of-semen-extenders-on-pre-freeze-shipped-semen

We share the summary on our website, courtesy of and with the permission of the International Society for Equine Reproduction.

(Zanlorenzi-Silva LP, Freitas-Dellโ€™Aqua CP, Baby MEA, Trinque CM, Frasson MS, Canuto LEF, Alvarenga MA, Dellโ€™Aqua JR JA, Cavalero TMS, Papa FO. The protective capacity of different diluents on stallion s***m during storage in a passive refrigeration system โ€œBotuflexโ“‡โ€ for 24 h at 5ยฐC before freezing. JEVS 145:105337 - abstract available on-line at https://www.sciencedirect.com/science/article/abs/pii/S0737080624003435)

๐๐‹๐„๐€๐’๐„ ๐๐Ž๐“๐„ - ๐’๐„๐‘๐•๐ˆ๐‚๐„๐’ ๐๐Ž๐“ ๐€๐•๐€๐ˆ๐‹๐€๐๐‹๐„!!Equine-Reproduction.com, LLC will be closed during the period August 19-24, 2026 i...
07/17/2026

๐๐‹๐„๐€๐’๐„ ๐๐Ž๐“๐„ - ๐’๐„๐‘๐•๐ˆ๐‚๐„๐’ ๐๐Ž๐“ ๐€๐•๐€๐ˆ๐‹๐€๐๐‹๐„!!

Equine-Reproduction.com, LLC will be closed during the period August 19-24, 2026 inclusive, as staff will be attending the ISEET's International Symposium on Equine Embryo Transfer and Technologies. Semen collection and shipping services for all resident and outside stallions - both fresh semen and frozen - will NOT be available during this time. We will be open again on August 25th.

๐’๐ญ๐š๐ฅ๐ฅ๐ข๐จ๐ง ๐ƒ๐ข๐ฌ๐ฆ๐จ๐ฎ๐ง๐ญ ๐‹๐š๐ญ๐ž๐ง๐œ๐ฒ ๐š๐ง๐ ๐๐ซ๐ž๐ ๐ง๐š๐ง๐œ๐ฒ ๐Ž๐ฎ๐ญ๐œ๐จ๐ฆ๐ž๐ฌ ๐ข๐ง ๐“๐ก๐จ๐ซ๐จ๐ฎ๐ ๐ก๐›๐ซ๐ž๐ ๐‡๐จ๐ซ๐ฌ๐ž๐ฌIn organized live cover situations, it's common p...
07/11/2026

๐’๐ญ๐š๐ฅ๐ฅ๐ข๐จ๐ง ๐ƒ๐ข๐ฌ๐ฆ๐จ๐ฎ๐ง๐ญ ๐‹๐š๐ญ๐ž๐ง๐œ๐ฒ ๐š๐ง๐ ๐๐ซ๐ž๐ ๐ง๐š๐ง๐œ๐ฒ ๐Ž๐ฎ๐ญ๐œ๐จ๐ฆ๐ž๐ฌ ๐ข๐ง ๐“๐ก๐จ๐ซ๐จ๐ฎ๐ ๐ก๐›๐ซ๐ž๐ ๐‡๐จ๐ซ๐ฌ๐ž๐ฌ

In organized live cover situations, it's common practice to take a "dismount sample" of ej******ed semen for evaluation, but how reliable are they, and what effect does the rapidity of the stallion dismounting after ej*******on have on pregnancy rates? This research paper presented at ISSR-8 produces some interesting results!

We share the summary on our website, courtesy of and with the permission of the International Society for Equine Reproduction: https://equine-reproduction.com/articles/issr-8/stallion-dismount-latency-and-pregnancy-rates

(Medica A, Griffin R, Swegen A, Gibb Z. (2025) Prolonged dismount latency and its relationship with pregnancy outcomes in Thoroughbred horses. JEVS 145:105304 - abstract available on-line at https://www.sciencedirect.com/science/article/abs/pii/S0737080624003101)

Storage of Thawed Frozen SemenFrozen semen is typically used within a short period of time following thawing, but there ...
07/06/2026

Storage of Thawed Frozen Semen
Frozen semen is typically used within a short period of time following thawing, but there are sometimes field conditions which preclude the ability to thaw or evaluated frozen semen adequately prior to use. This week's "Snippet" from the 8th International Symposium on Stallion Reproduction looks at possible management-level factors which could assist and improve thawing semen remotely from the mare, allowing it to then be transported to the site and inseminated.

We share the summary on our website, courtesy of and with the permission of the International Society for Equine Reproduction:

https://equine-reproduction.com/articles/issr-8/prolonged-storage-of-post-thaw-semen

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