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Medical Laboratory Science -MLS

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Medical Laboratory Science -MLS

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Medicine today is evidence-based and the laboratory is where over 85% of this evidence is obtained.

18/08/2026

My mood when I made a thin film for the first time:

07/08/2026

Gram positive bacilli, Classical lactobacilli a normal flora of the Va**na

14/04/2026

Urinalysis is the systemic examination of urine through physical, chemical and microscopic analysis to detect and assess the disorder in the renal and metabolic functions.

26/03/2026

Gram Staining (VERY COMMON IN VIVA)

Key Steps (Say this confidently in viva):
Prepare smear and air dry
Heat fix
Crystal violet (primary stain)
Iodine (mordant)
Decolorize (alcohol/acetone) ⚠️ critical step
Counterstain (safranin)
Observe under oil immersion

What Examiner Wants:
“Decolorization is the most critical step”
Gram + = purple, Gram – = pink
Based on cell wall thickness

Common Mistakes:
Over-decolorizing: Gram + appears Gram –
Thick smear = poor results

01/12/2025

What are the clinical significance of Albumin?

01/12/2025

Albumin is the major constituent of plasma protein (60%) which consist of single polypeptide chain of 585 amino acids with 17 disulphide bond.

What's the Normal conc of Albumin?

01/12/2025

Plasma protein is a blood component that contains anticoagulants and activated protein factors

What are the components of plasma protein?

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